论文部分内容阅读
[目的]探讨热休克蛋白HSP70-1+190(G/C)、HSP70-2+1267(A/G)两位点基因多态性与肺癌易感性的相关性。[方法]采用病例-对照研究,以病理确诊的肺癌患者159例为病例组,202名正常体检人群为对照组。应用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)技术检测HSP70-1+190(G/C)和HSP70-2+1267(A/G)的单核苷酸多态性。采用非条件Logistic回归分析两位点基因型与吸烟的交互作用及两位点之间的交互作用。[结果]HSP70-1+190(G/C)位点的3种基因型GG、GC和CC及G和C等位基因频率在病例组和对照组之间的分布,差异均有统计学意义(χ2=19.16,P<0.05;χ2=18.76,P<0.05),CC基因型与肺癌具有中等强度关联。HSP70-2+1267(A/G)位点的基因型及等位基因的分布在两组之间差异均无统计学意义(P>0.05)。按照吸烟情况进行分层分析发现,重度吸烟人群中携带HSP70-1+190(G/C)位点的GC基因型和GC+CC基因型者及HSP70-2+1267(A/G)位点的AA基因型的个体患肺癌的风险增高。基因型与吸烟的交互作用分析发现,HSP70-1+190(G/C)位点多态性和吸烟具有相乘交互作用(P=0.01)。HSP70-1+190(G/C)和HSP70-2+1267(A/G)两位点多态性之间存在交互作用(χ2=12.87,P=0.00)。[结论]HSP70-1+190(G/C)位点多态性可能与河南汉族人群中肺癌易感性有关,与HSP70-2+1267(A/G)和吸烟均具有相乘交互作用;HSP70-2+1267(A/G)位点多态性与肺癌的相关性还有待进一步研究,其AA基因型可能与重度吸烟之间存在交互作用。
[Objective] To investigate the relationship between the gene polymorphism of heat shock protein HSP70-1 + 190 (G / C) and HSP70-2 + 1267 (A / G) and lung cancer susceptibility. [Method] With case-control study, 159 patients with pathologically diagnosed lung cancer were selected as case group and 202 normal control subjects as control group. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect single nucleotide polymorphisms . Non-conditional logistic regression analysis was used to analyze the interaction between the two loci genotypes and smoking and the interaction between the two loci. [Results] The frequencies of GG, GC, CC, G and C alleles of HSP70-1 + 190 (G / C) loci were significantly different between the case group and the control group (χ2 = 19.16, P <0.05; χ2 = 18.76, P <0.05). There was a moderate correlation between CC genotype and lung cancer. The genotype and allele distribution of HSP70-2 + 1267 (A / G) loci had no significant difference between the two groups (P> 0.05). Stratified analysis according to the smoking status found that among the heavy smokers, those with GC genotype and GC + CC genotype carrying HSP70-1 + 190 (G / C) and HSP70-2 + 1267 (A / G) Individuals with AA genotype have a higher risk of developing lung cancer. Interaction analysis between genotypes and smoking found that HSP70-1 + 190 (G / C) polymorphism had a multiplicative interaction with smoking (P = 0.01). There was interaction between HSP70-1 + 190 (G / C) and HSP70-2 + 1267 (A / G) polymorphism (χ2 = 12.87, P = 0.00). [Conclusion] The polymorphism of HSP70-1 + 190 (G / C) locus may be related to the susceptibility to lung cancer in Henan Han population, and has a multiplicative interaction with HSP70-2 + 1267 (A / G) and smoking. HSP70 -2 + 1267 (A / G) locus polymorphism and lung cancer remains to be further studied, and its AA genotype may have an interaction with heavy smoking.