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目的探讨旱莲草对海马内注射Aβ1-40诱导的阿尔兹海默病(Alzheimer’s disease,AD)模型大鼠认知和炎症介导因子(IL-1β、IL-6和TNF-α)表达的影响。方法 SD大鼠随机分为对照组、模型组、旱莲草低剂量组、旱莲草高剂量组。右侧海马注射Aβ1-40构建AD模型大鼠,同时每天灌胃给予低剂量(8 g/kg)或高剂量(24 g/kg)的旱莲草,持续8周。Y迷宫测试大鼠的认知功能;酶联免疫法(enzyme-linked immuno sorbent assay,ELISA)检测IL-1β、IL-6和TNF-α的变化;HE染色观察大鼠前额叶皮质细胞形态改变。计量资料比较采用单因素方差分析,组间两两比较采用LSD-t检验,P<0.05为差异有统计学意义。结果四组大鼠电击总数[(35.08±10.40)、(54.36±9.41)、(44.18±8.76)、(36.17±8.67)次]、出错总数[(12.33±4.27)、(24.36±5.87)、(19.09±4.48)、(13.83±5.67)次]、记忆出错总数[(9.83±3.16)、(16.54±3.45)、(13.27±3.04)、(10.67±2.02)次]比较,差异均有统计学意义(均P<0.05)。AD模型组大鼠电击总数、出错总数及记忆出错总数均较对照组明显增多,差异均有统计学意义(均P<0.05)。四组大鼠IL-1β[(52.67±3.12)、(75.92±5.39)、(63.82±7.98)、(56.11±6.27)pg/ml]、IL-6[(43.04±6.17)、(67.68±7.52)、(52.71±5.76)、(46.78±4.08)pg/ml]、TNF-α[(82.55±6.02)、(137.08±9.40)、(100.12±8.47)、(87.03±6.56)pg/ml]水平比较,差异均有统计学意义(均P<0.05)。结论旱莲草可以改善AD大鼠的认知功能障碍,减轻脑组织的病理损伤,其机制可能与降低AD大鼠血清IL-1β、IL-6、TNF-α的过度表达有关。
Objective To investigate the effects of Eclipta prostrata on the expression of IL-1β, IL-6 and TNF-α in hippocampus of rats with Alzheimer’s disease (AD) induced by Aβ1-40 influences. Methods SD rats were randomly divided into control group, model group, low dose group of Eclipta prostrata, high dose group of Eclipta prostrata. The rats in the right hippocampus were injected with Aβ1-40 to establish AD model rats. The rats were orally administered with low dose (8 g / kg) or high dose (24 g / kg) of Eclipta prostrata for 8 weeks. Y maze was used to measure the cognitive function of rats. The changes of IL-1β, IL-6 and TNF-α were detected by enzyme-linked immunosorbent assay (ELISA). The morphological changes of prefrontal cortex were observed by HE staining . Measurement data were compared using one-way analysis of variance, the two groups compared using LSD-t test, P <0.05 for the difference was statistically significant. Results The total number of electric shock in the four groups was (35.08 ± 10.40), (54.36 ± 9.41), (44.18 ± 8.76) and (36.17 ± 8.67) times, respectively. The total number of errors was 12.33 ± 4.27 and 24.36 ± 5.87, 19.09 ± 4.48, and 13.83 ± 5.67, respectively), and the total number of memory errors were statistically significant (9.83 ± 3.16 vs. 16.54 ± 3.45, (13.27 ± 3.04), (10.67 ± 2.02), respectively] (All P <0.05). The total number of electric shocks, total number of errors and total number of memory errors in AD model group were significantly higher than those in control group (all P <0.05). IL-1β [(52.67 ± 3.12), (75.92 ± 5.39), (63.82 ± 7.98), (56.11 ± 6.27) pg / ml] and IL-6 [(43.04 ± 6.17), (67.68 ± 7.52 (52.71 ± 5.76), (46.78 ± 4.08) pg / ml], TNF-α [(82.55 ± 6.02), (137.08 ± 9.40), (100.12 ± 8.47), (87.03 ± 6.56) pg / ml] Comparison, the differences were statistically significant (P <0.05). Conclusion Eclipta prostrata can ameliorate cognitive dysfunction and reduce the pathological damage of brain tissue in AD rats. The mechanism may be related to the decrease of overexpression of IL-1β, IL-6 and TNF-α in AD rats.