论文部分内容阅读
目的 :研究核DNA数控定量分析技术对角结膜缘上皮性肿物病理诊断的意义 ,特别是进行早期组织病理学改变的诊断价值。方法 :应用自动图象分析技术及定量病理学方法 ,对 5 1例角结膜缘上皮性新生物 (上皮良性增生、上皮非典型性增生、原位癌、鳞状细胞癌 )定量测定核DNA含量和细胞核仁组成区嗜银蛋白 (argentumofnuclearorganizerregion ,AgNOR)含量。结果 :在四种不同病变中 ,细胞核DNA倍体含量 (2C ,3～ 4C ,≥ 5C)存在着显著的差异 ;AgNOR颗粒的分布形态、颗粒大小及所占核仁面积大小均存在着明显的差异 ,与病变的良、恶程度以及细胞的分化状况有着密切的关系。结论 :应用自动图象分析技术对角结膜缘上皮性肿物进行核DNA及AgNOR含量数控定量分析测定 ,可作为对组织病理学诊断可靠的辅助诊断方法 ,进一步分析了解增殖性病变的细胞增殖活性 ,评价其早期增殖分化潜势 ,鉴别良恶性病变 ,有利于从细胞生物学特点评估患者的预后 ,具有一定的实用意义。
Objective: To study the significance of numerical quantitative nuclear DNA analysis in the pathological diagnosis of epithelial conjunctiva epithelial tumors, especially for the early diagnosis of histopathological changes. Methods: Quantitative detection of nuclear DNA content in 51 epithelial neoplasms (benign epithelial hyperplasia, epithelial atypical hyperplasia, carcinoma in situ and squamous cell carcinoma) by using automatic image analysis technique and quantitative pathology method And the content of argentumofnuclearorganizerregion (AgNOR) in the nucleolus. Results: There were significant differences in DNA ploidy (2C, 3 ~ 4C, ≥ 5C) among the four different pathological changes. The distribution pattern, particle size and nucleolar area of AgNOR particles were significantly different Differences, and the lesion of good and evil, as well as the degree of differentiation of cells has a close relationship. Conclusion: Quantitative quantitative analysis of nuclear DNA and AgNOR content in the limbal conjunctival epithelial tumor using automatic image analysis technique can be used as a reliable diagnostic method for histopathological diagnosis, and further analysis of cell proliferative activity of proliferative lesions , To evaluate its early proliferation and differentiation potential, to identify benign and malignant lesions, is conducive to assessing the prognosis of patients from the characteristics of cell biology, has a certain practical significance.