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本文介绍一种半定量的尿β_2m酶联免疫抑制试验,方法简便、快速,适于临床常规使用。应用该方法对40例正常人、40例正常小儿、74例肾病小儿、91例成人肾病患者、224例老年人(50～100岁)、214例正常孕妇以及46名妊娠中毒症孕妇尿液中β_2m进行测定。结果提示,尿β_2m含量变化可作为肾小管疾病的鉴别诊断指标之一,并可估计肾脏老年性变及妊娠中肾脏受损的一个灵敏的先兆现象。本文对各种实验参数进行了讨论。
This article describes a semi-quantitative urinary β_2m ELISA test, the method is simple and rapid, suitable for clinical routine use. In this study, 40 normal controls, 40 normal children, 74 nephrotic children, 91 adult nephropathy patients, 224 elderly patients (50-100 years old), 214 normal pregnant women and 46 pregnant women with gestational toxicosis β_2m. The results suggest that urinary β_2m content changes can be used as a differential diagnosis of tubulointerstitial disease, one of the indicators, and can be estimated in the elderly with renal aging and gestational kidney damage a sensitive precursor phenomenon. This article discusses various experimental parameters.