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目的用心肌匀浆建立心肌肌浆网钙释放通道检测方法，并观察其在高血压中的变化。方法用３Ｈ标记兰尼定（ｒｙａｎｏｄｉｎｅ），探索在大鼠心肌匀浆中建立钙释放通道放射配基分析的最佳条件，并对自发性高血压大鼠（ＳＨＲ）左室心肌钙释放通道进行饱和实验。结果①保温时间在０～６０分钟内，３Ｈｒｙａｎｏｄｉｎｅ与心肌钙释放通道结合量迅速增加，９０分钟达平衡；匀浆蛋白在０～４００μｇ，３Ｈｒｙａｎｏｄｉｎｅ结合量与蛋白质浓度呈直线关系；反应介质中游离钙浓度直接影响３Ｈｒｙａｎｏｄｉｎｅ的结合量。②ＳＨＲ及正常对照组（ＷＫＹ）标准饱和曲线及Ｓｃａｔｃｈａｒｄ作图显示，３Ｈｒｙａｎｏｄｉｎｅ的特异性结合高，呈单位点结合等特点，ＳＨＲ组最大结合量（Ｂｍａｘ）值明显高于ＷＫＹ组（Ｐ＜００１）。结论用３Ｈｒｙａｎｏｄｉｎｅ可在心肌匀浆中进行钙释放通道放射配基分析，且发现３ＨｒｙａｎｏｄｉｎｅＢｍａｘ升高与高血压左室肥厚密切相关。
Objective To establish a method for detection of calcium release channel in cardiac muscle sarcoplasmic reticulum with myocardial homogenate and observe its changes in hypertension. Methods 3H-labeled ryanodine was used to explore the optimal conditions for establishing radioligand analysis of calcium release channel in rat myocardial homogenate. The left ventricular calcium channel in spontaneously hypertensive rats (SHR) Saturation experiment. Results ①In the period of 0 ~ 60min, the binding quantity of 3Hryanodine and calcium channel in myocardium rapidly increased, reaching a balance in 90 minutes. The binding protein in the range of 0 ~ 400μg and 3Hryanodine showed a linear relationship with the protein concentration; The concentration of free calcium in the medium directly affects the binding amount of 3Hryanodine. (2) The standard saturation curve and Scatchard plot of SHR and normal control group (WKY) showed that 3Hryanodine had high specific binding and single site binding, and the maximum binding capacity (Bmax) in SHR group was significantly higher than that in WKY group (P < 0 01). Conclusion 3Hryanodine can be used in myocardial homogenate calcium release channel radioligand analysis, and found that 3H yananodineBmax increased hypertension and left ventricular hypertrophy are closely related.