Solid-State ~(13)C Nuclear Magnetic Resonance Spectroscopic Characterization of Soil Organic Matter

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Forest management practices such as prescribed burning and thinning in forest ecosystems may alter the properties of soil organic matter(SOM). In this study, surface soils from field plots in the Bankhead National Forest, Alabama, USA, were used to investigate possible SOM transformations induced by thinning and burning. Elemental analysis and solid-state ~(13)C cross polarization magic angle spinning nuclear magnetic resonance(~(13)C CPMAS NMR) spectroscopy were used to characterize SOM fractions in whole soils, humic substances, and density fractions. Our data revealed that the changes in SOM fractions due to the repeated burning carried out in the forest ecosystem studied were involved mainly with alkyl C, O-alkyl C, and carbohydrate functional groups, implying that most prominent reactions that occurred involved dehydrogenation, de-oxygenation, and decarboxylation. In addition, burning and thinning might have also affected the distribution and composition of free and occluded particulate SOM fractions. The limited structural changes in SOM fractions suggested that low-intensity prescribed fire in the forest ecosystem studied will not create major structural changes in SOM fractions. Forest management practices such as describing burning and thinning in forest ecosystems may alter the properties of soil organic matter (SOM). In this study, surface soils from field plots in the Bankhead National Forest, Alabama, USA, were used to investigate possible SOM transformations induced by thinning and burning. Elemental analysis and solid-state ~ (13) C cross polarization magic angle spinning nuclear magnetic resonance (~ (13) C CPMAS NMR) spectroscopy were used to characterize SOM fractions in whole soils, humic substances, and density fractions. Our data revealed that the changes in SOM fractions due to the repeated burning carried out in the forest ecosystem studied were involved mainly with alkyl C, O-alkyl C, and carbohydrate functional groups, implying that most prominent reactions that occurred in dehydrogenation, de-oxygenation, and decarboxylation. In addition, burning and thinning might also also affected the distribution and composition of free and occluded parti culate SOM fractions. The limited structural changes in SOM fractions suggested that low-intensity prescribed fire in the forest ecosystem studied will not create major structural changes in SOM fractions.
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