论文部分内容阅读
目的 探讨大鼠不同年龄组来源的神经干细胞体外培养和诱导分化的最佳条件。 方法 分离 12～ 15d大鼠胚胎、1～ 2d新生鼠的脑组织和成年大鼠脑的海马区和嗅球 ,在条件培养基 (包含生长因子 )中增殖培养 ,用神经干细胞的特异性单克隆抗体巢蛋白 (nestin)鉴定克隆细胞 ;去除生长因子 ,降低血清浓度到 2 %后 ,诱导细胞分化 ,用神经细胞的特异性单克隆抗体鉴定分化细胞。 结果 获得了大量可自我增殖并分化为 3种神经细胞 (神经元 ,星形胶质细胞 ,少突胶质细胞 )的神经前体细胞。 结论 在适当的分离培养条件下 ,可以获得大量的神经前体细胞 ,为神经干细胞的基础和临床研究提供细胞基础
OBJECTIVE: To investigate the optimal conditions for the differentiation of neural stem cells from different age groups in vitro. Methods The brain tissues of 12 ~ 15 d rat embryos, 1 ~ 2 d neonatal rat brain and hippocampus and olfactory bulb of adult rat brain were isolated and cultured in conditioned media (including growth factors). The specific monoclonal antibodies against neural stem cells Nestin was used to identify the cloned cells. After removing the growth factors and reducing the serum concentration to 2%, the cells were induced to differentiate, and the differentiated cells were identified with specific monoclonal antibodies against neurons. As a result, a large number of neural progenitor cells that proliferated and differentiated into three types of neurons (neurons, astrocytes, and oligodendrocytes) were obtained. Conclusion Under appropriate culture conditions, a large number of neural progenitor cells can be obtained and provide the basic of cells for basic and clinical research of neural stem cells