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目的观察反复高热惊厥(FS)大鼠脑组织核因子-κB(NF-κB)的表达,探讨NF-κB在FS大鼠脑损伤发病中的意义。方法雄性SD大鼠51只按随机数字法分为正常对照组(n=14)、高热对照组(n=19)和FS组(n=18)。热水浴法建立FS模型;予热水浴而未出现FS为高热对照组;正常对照组未予任何处理。免疫组织化学检测NF-κB在各组大鼠脑组织各区的表达,原位末端标记法检测各组大鼠脑组织细胞凋亡。采用SPSS13.0软件进行统计学处理。结果FS组大鼠脑组织各区NF-κB阳性细胞数明显高于正常对照组和高热对照组(Pa<0.01);高热对照组大鼠脑组织各区NF-κB阳性细胞数也明显高于正常对照组(Pa<0.01)。FS组大鼠脑组织各区NF-κB阳性细胞数从高到低依次为海马齿状回[(26.10±2.40)/HP]、CA3区[(24.30±2.0)/HP]、CA1区[(22.50±3.98)/HP]、颞叶皮层[(22.10±2.33)/HP],这种部位差异与原位末端标记相一致,凋亡指数从高到低依次为海马齿状回(32.65±2.14),CA3区(28.99±1.16),CA1区(24.28±0.92),颞叶皮层(22.19±1.06)。结论反复FS致脑损伤后,NF-κB在脑组织中的表达存在部位差异,且对神经细胞的凋亡起重要作用。
Objective To observe the expression of nuclear factor-κB (NF-κB) in the brain tissue of rats with repeated febrile seizures (FS) and to explore the significance of NF-κB in the pathogenesis of brain injury in rats. Methods Fifty - one male Sprague - Dawley rats were randomly divided into normal control group (n = 14), hyperthermia control group (n = 19) and FS group (n = 18). Hot water bath method to establish FS model; to hot water bath without FS high fever control group; normal control group without any treatment. Immunohistochemistry was used to detect the expression of NF-κB in various regions of rat brain tissue. Apoptosis of brain tissue in each group was detected by in situ end-labeling. Using SPSS13.0 software for statistical analysis. Results The number of NF-κB positive cells in brain tissue of FS group was significantly higher than that of normal control group and hyperthermia control group (Pa <0.01). The number of NF-κB positive cells in brain tissue of FSH group was also significantly higher than that of normal control group Group (Pa <0.01). The number of NF-κB positive cells in hippocampus of hippocampus in FS group was (26.10 ± 2.40) HP, CA3 [24.30 ± 2.0] / HP, CA1 [[22.50 ± 3.98) / HP], and temporal lobe cortex [(22.10 ± 2.33) / HP]. The difference of this site was consistent with the in situ terminal labeling. The apoptotic index was hippocampal dentate gyrus from high to low (32.65 ± 2.14) , CA3 (28.99 ± 1.16), CA1 (24.28 ± 0.92) and temporal cortex (22.19 ± 1.06). Conclusions After repeated FS induced brain injury, the expression of NF-κB in the brain tissue is different and plays an important role in the apoptosis of neural cells.