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研究分别以2种大豆WDD00172(WDD-172)和晋豆21(JD-21)为实验材料,探讨干旱胁迫对不同品种大豆下胚轴生理特性及抗氧化酶活性的影响。结果表明,随着9%PEG6000胁迫时间的延长,大豆下胚轴MDA含量和离子渗漏显著增高,且WDD-172增幅较大;干旱胁迫下GSH含量、CAT和POD活性持续下降,APX活性则先上升后下降,而且WDD-172下降更加明显。研究发现JD-21更加抗旱可能与体内高浓度的葡萄糖-6-磷酸脱氢酶(G6PDH)活性有关;干旱胁迫下JD-21比WDD-172更加耐受干旱胁迫,可能是由于体内抗氧化酶活性(CAT、POD和APX)和抗氧化物质GSH含量较高的原因。
Two soybean WDD00172 (WDD-172) and Jin-Dou 21 (JD-21) were used as experimental materials to study the effects of drought stress on the physiological characteristics and antioxidant enzyme activities of different hypocotyls of soybean. The results showed that the content of MDA and ion leakage in hypocotyls of soybean increased significantly with the prolongation of 9% PEG6000 stress, and the increase of WDD-172 was significant. The activities of GSH, CAT and POD decreased continuously under drought stress, while the activities of APX First rise and then decline, and WDD-172 decline more pronounced. The results showed that more drought-resistant JD-21 may be related to high concentration of glucose-6-phosphate dehydrogenase (G6PDH) in vivo. Under drought stress, JD-21 is more resistant to drought stress than WDD-172, Activity (CAT, POD and APX) and antioxidant GSH content higher reasons.