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OBJECTIVE To evaluate the REGγ expression in gastric cancertissue and gastric cancer cell lines of various differentiation levelsand its clinical significance.METHODS Immunohistochemistry was used to detect theexpression of REGγ protein in 70 specimens of gastric cancer and30 specimens of normal gastric mucosa. The relationship betweenthe expression of REGγ protein and the biological behaviors ofgastric cancer was analyzed. RT-PCR and Western blot were usedto detect the mRNA level and the protein expression of REGγ innormal gastric cell line GES-1, well differentiated gastric cancercell line MKN-28, moderately differentiated gastric cancer cellline SGC-7901 and poorly differentiated gastric cancer cell lineBGC-823.RESULTS The expression rate of REGγ protein in gastric cancertissue (52/70, 74.29%) was significantly higher than that in normalgastric tissue (12/30, 40%) (P < 0.01). The expression rate of REGγwas correlated with tumor size (P < 0.01), lymph node metastasis (P< 0.05), differentiation degree (P < 0.01), infiltration depth (P < 0.01)and distant metastasis (P < 0.05). RT-PCR analysis showed that theexpression of REGγ mRNA was 0.459 ± 0.079 in the normal gastricmucosa cell line, 0.588 ± 0.118 in the well differentiated gastriccancer cell line, 0.715 ± 0.066 in the moderately differentiatedgastric cancer cell line, and 0.873 ± 0.099 in the poorlydifferentiated gastric cancer cell line, showing a negative correla-tion between REGγ mRNA expression and differentiation level (P< 0.05). Western blot analysis showed that the expression of REGγprotein was 0.712 ± 0.065 in the normal gastric mucosa cell line,1.176 ± 0.185 in the well differentiated gastric cancer cell line, 1.533± 0.127 in the moderately differentiated gastric cancer cell line, and2.061 ± 0.398 in the poorly differentiated gastric cancer cell line,showing a negative correlation between REGγ protein expressionand differentiation level (P < 0.05).CONCLUSION REGγ is expressed in gastric cancer tissue andnormal gastric tissue. In gastric cancer tissues, REGγ expression ispositively correlated with the tumor size, lymph node metastasis,differentiation degree, infiltration depth and distant metastasis.Detecting the expression of REGγ mRNA and protein is helpfulfor early diagnosis and predicting prognosis of gastric cancer.
OBJECTIVE To evaluate the REGγ expression in gastric cancertissue and gastric cancer cell lines of various differentiation levels and its clinical significance. METHODS Immunohistochemistry was used to detect the expression of REGγ protein in 70 specimens of gastric cancer and 30 specimens of normal gastric mucosa. The relationship betweenthe expression of REG [gamma] protein and the biological behaviors of gastric cancer were analyzed. RT-PCR and Western blot were used to detect the mRNA level and the protein expression of REG [gamma] innormal gastric cell line GES-I, well differentiated gastric cancer cell line MKN-28, moderately differentiated gastric cancer Cell line SGC-7901 and poorly differentiated gastric cancer cell line BGC-823 .RESULTS The expression rate of REGγ protein in gastric cancertissue (52/70, 74.29%) was significantly higher than that in normal gastric tissue (12/30, 40%) <0.01). The expression rate of REGγwas correlated with tumor size (P <0.01), lymph node metastasis (P <0.05) RT-PCR analysis showed that the expression of REGγ mRNA was 0.459 ± 0.079 in the normal gastric mucosa cell line, 0.588 ± 0.118 in the control group (P <0.01) well differentiated gastriccancer cell line, 0.715 ± 0.066 in the moderately differentiated gastric cancer cell line, and 0.873 ± 0.099 in the poorly differentiated gastric cancer cell line, showing a negative correlation between REGγ mRNA expression and differentiation level (P <0.05). Western blot analysis showed that the expression of REGγprotein was 0.712 ± 0.065 in the normal gastric mucosa cell line, 1.176 ± 0.185 in the well differentiated gastric cancer cell line, 1.533 ± 0.127 in the moderately differentiated gastric cancer cell line, and2.061 ± 0.398 in the poorly differentiated gastric cancer cell line, showing a negative correlation between REGγ protein expression and differentiation level (P <0.05) .CONCLUSION REGγ is expressed in gastr ic cancer tissue andnormal gastric tissue. In gastric cancer tissues, REGγ expression ispositively correlated with the tumor size, lymph node metastasis, differentiation degree, infiltration depth and distant metastasis. Detection of the expression of REGγ mRNA and protein is helpful for early diagnosis and predicting prognosis of gastric cancer.