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AIM:To investigate the antitumor activities of tachyplesinon human hepatocellular carcinoma(HCC)cells.METHODS:Tachyplesin,isolated from acid extracts ofChinese horseshoe crab(Tachypleus tndentatus)hemocytes,was used to treat the human HCC cell line SMMC-7721.Effectsof tachyplesin on the proliferation of SMMC-7721 cells weremeasured with trypan blue dye exclusion test and HE staining.The morphology and ultrastructure of the cells were examinedby light microscopy and transmission electron microscopy,respectively.The activities of γ-glutamyltransferase(γ-GT)andtyrosine aminotransferase(TAT)were assayed withbiochemical methods.The levels of alpha fetoprotein(α-FP),proliferating cell nuclear antigen(PCNA),p21~(WAF1/CIP1)and c-myc were examined by immunocytochemistry.RESULTS:After treatment with tachyplesin 3.0 mg/L,theproliferation of SMMC-7721 cells was inhibited significantly,with the cell growth inhibitory rate amounted to 55.57 % andthe maximum cell mitotic index declined by 43.68 %.Themorphology and ultrastructure underwent restorationalalteration.The activity of γ-GT declined while TAT activityincreased obviously,and the levels of α-FP and PCNA decreased.Moreover,the expression of p21~(WAF1/CIP1)protein was up-regulated and that of c-myc protein was down-regulated.CONCLUSION:Tachyplesin could effectively inhibit theproliferation of hepatocarcinoma cells,reverse the malignantmorphological and ultrastructural characteristics,alter thelevels of enzymes and antigens,regulate the expression ofdifferentiation-associated oncogene and tumor suppressorgene,and induce hepatocarcinama cell differentiation.
AIM: To investigate the antitumor activities of tachyplesinon human hepatocellular carcinoma (HCC) cells.METHODS: Tachyplesin, isolated from acid extracts of Chinese horseshoe crab (Tachypleus tndentatus) hemocytes, was used to treat the human HCC cell line SMMC-7721.Effects of tachyplesin on The proliferation of SMMC-7721 cells weremeasured with trypan blue dye exclusion test and HE staining.The morphology and ultrastructure of the cells were examined by light microscopy and transmission electron microscopy,respectively.The activities of γ-glutamyltransferase(γ-GT)andtyrosine aminotransferase( TAT) were assayed with biochemical methods.The levels of alpha fetoprotein(α-FP), proliferating cell nuclear antigen(PCNA), p21~(WAF1/CIP1)and c-myc were examined by immunocytochemistry.RESULTS:After treatment with tachyplesin 3.0 mg /L,theproliferation of SMMC-7721 cells was inhibited significantly,with the cell growth inhibitory rate amounted to 55.57 % andthe maximum cell mitotic index declined by 43.68 %. Themorphology and ultrastructure underwent restorationalalteration.The activity ofγ-GT declined while TAT activityincreased obviously, and the levels of α-FP and PCNA decreased.Moreover, the expression of p21~(WAF1/CIP1)protein was up-regulated and that of c -myc protein was down-regulated.CONCLUSION:Tachyplesin could effectively inhibit the proliferation of hepatocarcinoma cells, reverse the malignant morphological and ultrastructural characteristics, alter the levels of enzymes and antigens,regulate the expression of differential-associated oncogene and tumor suppressorgene, and induce hepatocarcinoma cell differentiation.