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目的对新疆巴音郭楞蒙古自治州(巴州)地区手足口病例进行核酸检测,为手足口病疫情防控提供科学依据。方法采用实时荧光PCR(real-time-PCR)法检测粪便、肛拭子标本中的手足口病毒核酸。结果 2010年共检测106份手足口病标本,阳性78份,阳性率73.58%;阳性标本中,EV71型占42.31%、CVA16型占38.46%、其他肠道病毒占19.23%,9例重症病例均为EV71型;5份为肛拭子标本检测结果均为阴性,其余均为粪便标本。结论 2010年巴州手足口病检测结果显示EV71型占优势,可解释巴州地区重症病例在全疆较多的原因;为更好监测手足口病病原学变化,采样应首选疱疹液或粪便标本。
Objective To detect nucleic acid in hand, foot and mouth cases of Bayinguoleng Mongol Autonomous Prefecture (Bazhou) in Xinjiang in order to provide a scientific basis for prevention and control of hand-foot-mouth disease. Methods Real-time-PCR method was used to detect hand-foot-mouth virus nucleic acid in fecal and anal swab specimens. Results A total of 106 samples of hand-foot-mouth disease were detected in 2010, with a positive rate of 73.58%. In positive samples, EV71 accounted for 42.31%, CVA16 accounted for 38.46%, other enteroviruses accounted for 19.23%, and 9 severe cases For the EV71 type; 5 copies of the anal swab specimens were negative test results, the rest are stool specimens. Conclusion The results of hand-foot-mouth disease test in Bazhou in 2010 showed that EV71 predominates, which may explain the reasons why there are more severe cases in Bazhou in Xinjiang. To better monitor the etiology of hand-foot-mouth disease, samples should be herpes fluid or stool samples .