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我们应用梨形四膜虫,按毒理学方法,以繁殖抑制为判断指标,从细胞水平上对汞、镉、砷、锌、氰化物五种毒物进行了联合作用毒性的探讨,报告如下。 材料和方法 所用细胞株为北京大学生物系提供的无菌梨形四膜虫细胞单株[S_1] 。 细胞培养采用液相培养,培养液配方:胰蛋白胨0.5％,多胨0.5％ 葡萄糖0.25％,用水为去离子水。培养温度27.5±0.5℃,pH值7.0±0.2,培养时间48小时。全部实
We applied Tetrahymena pear, according to toxicological methods to reproductive inhibition as a judge indicators, from the cellular level of mercury, cadmium, arsenic, zinc, cyanide poisoning of five joint toxicity study, the report is as follows. MATERIALS AND METHODS Cell lines used were the sterile Tetrahymena tetraticroblasts cells supplied by the Peking University Department of Biology [S_1]. Cell culture using liquid culture medium formula: tryptone 0.5%, polypeptone 0.5% glucose 0.25%, water for the deionized water. Culture temperature 27.5 ± 0.5 ℃, pH value 7.0 ± 0.2, culture time 48 hours. All real