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目的:建立跌打丸中血竭素和姜黄素的UPLC含量测定方法。方法:采用超高效液相色谱法,色谱柱为Waters Acquity UPLC BEH C_(18)(100 mm×2.1 mm,1.7μm)柱,流动相为乙腈-0.05 mol·L~(-1)磷酸二氢钠溶液(50∶50),血竭素检测波长为440 nm,姜黄素检测波长为431 nm,柱温为30℃,流速为0.1 ml·min~(-1)。结果:血竭素在0.001 8~0.036 4μg(r=0.999 9),姜黄素在0.000 8~0.015 6μg(r=0.999 9)之间呈良好的线性关系。血竭素平均加样回收率为97.94%(RSD=0.89%),姜黄素平均加样回收率为98.45%(RSD=0.91%)。结论:该方法简便快速,重复性好,可用于跌打丸中血竭素和姜黄素的含量测定。
Objective: To establish a method for determination of UPLC content of dracorhodin and curcumin. Methods: The column was Waters Acquity UPLC BEH C 18 (100 mm × 2.1 mm, 1.7 μm) column with a mobile phase of acetonitrile-0.05 mol·L -1 dihydrogen phosphate (50:50). The detection wavelength of Doxorubicin was 440 nm. The detection wavelength of curcumin was 431 nm. The column temperature was 30 ℃ and the flow rate was 0.1 ml · min ~ (-1). Results: The results showed that there was a good linear relationship between the concentration of dracorhodin and the curcumin at 0.0008-0.036 μg (r = 0.999 9) and 0.0008-0.015 6 μg (r = 0.999 9). The average recovery rate of DRX was 97.94% (RSD = 0.89%). The average recoveries of curcumin were 98.45% (RSD = 0.91%). Conclusion: The method is simple, rapid and reproducible. It can be used to determine the content of dracorhodin and curcumin in Beidai Wan.