论文部分内容阅读
目的研究环孢素A对免疫功能的影响。方法通过2,4-二硝基氟苯(DNFB)诱导小鼠迟发型变态反应考察该药对细胞免疫功能的影响;用L929作为靶细胞的MTT染色法测定该药对小鼠腹腔巨噬细胞经脂多糖(LPS)刺激产生肿瘤坏死因子α(TNF-α)的影响。结果环孢素A能明显抑制DNFB诱导的小鼠迟发型变态反应;高剂量能促进小鼠腹腔巨噬细胞TNF-α生成。结论环孢素A有较强的免疫抑制作用。
Objective To study the effect of cyclosporin A on immune function. Methods The delayed-type allergic reaction induced by 2,4-dinitrofluorobenzene (DNFB) was used to investigate the effect of this drug on cellular immune function. MTT assay was used to determine the effect of this drug on mouse peritoneal macrophages Effect of lipopolysaccharide (LPS) on the production of tumor necrosis factor alpha (TNF-α). Results Cyclosporine A significantly inhibited DNFB-induced delayed type hypersensitivity in mice. High dose of cyclosporin A could promote the production of TNF-α in mouse peritoneal macrophages. Conclusion Cyclosporin A has a strong immunosuppressive effect.