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由玉米矮花叶病毒(MDMV)引起的玉米矮花叶病日趋严重,利用抗病品种是防治该病的根本措施。研究玉米抗病机理是快速培育抗病品种的基础,国外曾用免疫荧光法检测MDMV在接种叶片上的侵染和扩展。该法定位效果好,但对于叶片有较厚角质层的禾本科作物需要酶消解...
Maize dwarf mosaic disease caused by maize dwarf mosaic virus (MDMV) is becoming more and more serious. The use of resistant varieties is the fundamental measure to prevent this disease. Studying the mechanism of disease resistance in maize is the basis for the rapid development of resistant varieties. In foreign countries, the infection and expansion of MD-MV on inoculated leaves have been detected by immunofluorescence assay. The method of positioning effect is good, but thicker cuticle for the leaves of grassy crops require enzymatic digestion ...