论文部分内容阅读
目的探讨哮喘婴幼儿肠道菌群构成特征与细胞免疫功能的相关性。方法选取35例哮喘急性发作期婴幼儿,定量PCR检测哮喘婴幼儿肠道菌群构成,采用流式细胞术细胞内染色技术检测Thl/Th2细胞。结果哮喘婴幼儿粪便中专性厌氧菌双歧杆菌、乳酸杆菌的含量明显少于健康对照组,而大肠杆菌含量多于对照组(P<0.05);Thl/Th2平衡呈现向Th2偏移趋势,与对照组比较,[(2.01±0.96)%vs.(9.78±1.97)%]差异有统计学意义(P<0.05)。双歧杆菌与大肠杆菌比值(B/E值)与Th1/Th2比例呈正相关。结论哮喘婴幼儿细胞免疫异常可能与肠道菌群失衡相关。
Objective To investigate the relationship between intestinal microflora and cellular immune function in infants with asthma. Methods Thirty-five infants and young children with acute exacerbation of asthma were selected. The intestinal microflora was determined by quantitative PCR. Th1 / Th2 cells were detected by flow cytometry. Results The contents of Bifidobacteria and Lactobacilli in the stool of asthmatic infants and young children were significantly less than those in the control group (P <0.05), while the Thl / Th2 balance showed a trend toward Th2 Compared with the control group, the difference was statistically significant (P <0.05) [(2.01 ± 0.96)% vs (9.78 ± 1.97)%]. Bifidobacterium and Escherichia coli ratio (B / E value) and Th1 / Th2 ratio was positively correlated. Conclusion The abnormal cellular immunity in infants may be related to imbalance of intestinal microflora.