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目的比较三种检测AmpC酶的方法,以找出一种能简便、准确、适合临床常规应用的检测AmpC酶的方法。同时了解我院临床分离菌株AmpC酶的产生情况。方法采用酶提取物三维试验、头孢西丁三相试验及氯唑西林双纸片协同法同时检测75株阴沟肠杆菌和55株鲍曼不动杆菌的AmpC酶产生情况。结果用酶提取物三维试验检测到所试阴沟肠杆菌和鲍曼不动杆菌的AmpC酶阳性株分别为22株(占29·3%)和10株(占18·2%)、头孢西丁三相试验及氯唑西林双纸片协同法与酶提取物三维试验的符合率分别为70·8%(92/130)和94·6%(123/130)。结论头孢西丁三相试验检测AmpC酶存在严重的假阳性,而氯唑西林双纸片协同法结果可靠、方法简便,适合于临床常规应用。
Objective To compare three methods of detecting AmpC enzyme in order to find out a method to detect AmpC enzyme which is simple, accurate and suitable for clinical routine application. At the same time to understand our hospital clinical isolates AmpC enzyme production. Methods The three-dimensional enzymatic extraction test, three-phase cefoxitin test and cloxacillin bi-paper synergistic method were used to detect the AmpC enzyme production in 75 strains of Enterobacter cloacae and 55 strains of Acinetobacter baumannii simultaneously. Results AmpC enzyme positive strains of Enterobacter cloacae and Acinetobacter baumannii were detected as 22 strains (29.3%) and 10 strains (18.2%) respectively by three-dimensional test of enzyme extracts. Cefoxitin The coincidence rates of three-phase test and three-dimensional test of cloxacillin double-sheet synergistic method with enzyme extract were 70.8% (92/130) and 94.6% (123/130) respectively. Conclusion Cefoxitin three-phase test for the detection of AmpC enzyme serious false positive, and cloxacillin double paper synergy results reliable, the method is simple and suitable for routine clinical applications.