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选用45个分布于10条染色体上的DNA探针,结合4种限制性内切酶,检测了广亲和品种Pecos和测验种南京11、秋光之间DNA限制性片段长度多态性(RFLP),发现其中15个探针能在Pecos、南京11和秋光之间表现多态性。进一步选用14个表现多态性的探针和色素原基因C分析了三交群体(Pecos/南京11∥秋光)中单株结实率的分离,结果表明第6染色体上的RFLP标记RG138、RG64、RG456以及色素原基因C与籼粳杂交亲和性有显著的连锁关系;位于第12染色体上的标记RG81也与三交群体单株结实率显著相关。根据标记与性状的共分离资料,初步绘制了第6染色体上该籼粳杂交亲和性座位的RFLP连锁图。此外,还发现第12染色体上的两个标记RG81和RG323在三交群体中两种基因型的分离比例不符合1:1。
45 DNA probes distributed on 10 chromosomes and 4 restriction enzymes were used to detect the DNA restriction fragment length polymorphism (RFLP) between Pecos, a broad-compatible cultivar, and Nanjing 11, And found that 15 of them showed polymorphism between Pecos, Nanjing 11 and autumn light. Fourteen polymorphic probes and procollagen C were selected to analyze the segregation of seed setting rate in the three cross population (Pecos / Nanjing 11∥Auguang). The results showed that the RFLP markers RG138, RG64, There was a significant linkage between RG456 and the progeny of C and indica-japonica hybridization. The marker RG81 located on chromosome 12 was also significantly associated with the seed setting rate of the three cross population. According to the co-segregation data of markers and traits, the RFLP linkage map of compatibility loci on indica-japonica hybrids on chromosome 6 was preliminary mapped. In addition, it was also found that the two markers RG81 and RG323 on chromosome 12 did not match the 1: 1 ratio of the two genotypes in the tri-crosses.