论文部分内容阅读
目的 :观察补肾益智方对由Aβ片段毒性诱导的NG1 0 8- 1 5细胞老年性痴呆 (AD)模型的影响。方法 :利用细胞计数、MTT及流式细胞仪测定等方法观察补肾益智方含药血清处理由Aβ片段毒性诱导的NG1 0 8- 1 5细胞存活率、形态学及细胞周期的改变。结果 :补肾益智方含药血清能增加Aβ片段毒性诱导的NG1 0 8- 1 5细胞存活率和细胞突起 ;细胞增殖周期分析显示 ,含药血清能增加S期细胞数。结论 :补肾益智方具有减轻细胞对Aβ的神经毒性反应 ,表明该方通过拮抗AD的病理发展而发挥对AD的治疗作用
Objective: To observe the effect of Bushen Yizhi Recipe on senile dementia (AD) model of NG10-57 cells induced by Aβ fragment toxicity. METHODS: The cell viability, morphology and cell cycle of NG10-57 cells induced by Aβ-fragment toxicity were observed by cell counting, MTT and flow cytometry. Results : Bushen Yizhi Recipe containing serum can increase the survival rate and cell protrusion of NG10-85 cells induced by Aβ fragment toxicity. Cell proliferation cycle analysis showed that drug-containing serum can increase the number of cells in S phase. Conclusion: Bushen Yizhi Decoction can reduce the neurotoxicity of cells to Aβ, indicating that this side can play a therapeutic role in AD by antagonizing the pathological development of AD.