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目的探索溴氰菊酯(DM)对大鼠肝脏的氧化损伤作用及其作用机制。方法将大鼠随机分为8组(每组8只),7 d染毒分别为DM 3.12 mg/kg组﹑DM 6.25 mg/kg组、DM 12.50 mg/kg组及对照组,连续染毒7 d。24周染毒分别为DM 1.56 mg/kg组﹑DM 3.12 mg/kg组﹑DM 6.25 mg/kg组及对照组,连续染毒5 d停2 d,染毒时间为24周。检测DM对大鼠肝脏丙二醛(MDA)、一氧化氮(NO)、一氧化氮合酶(NOS)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(CSH-PX)、超氧化物歧化酶(SOD)、总抗氧化能力(T-AOC)活性的影响。结果 7 d染毒3个染毒组与对照组比较,P>0.05差别没有统计学意义。24周染毒各染毒组与对照组比较,MDA、NO均升高,SOD、CAT、GSH-PX、T-AOC活性均降低,P<0.05差别有统计学意义,并产生随剂量变化的趋势。结论 DM经过长期摄入可引起大鼠肝脏中MDA、NO、CAT、T-AOC、CSH-PX、SOD活性的改变,导致抗氧化能力下降,对肝脏造成氧化损害。
Objective To explore the effect of deltamethrin on rat liver oxidative damage and its mechanism. Methods The rats were randomly divided into 8 groups (8 in each group). The rats in the 7 d groups were exposed to DM 3.12 mg / kg, DM 6.25 mg / kg, DM 12.50 mg / kg and control group for 7 consecutive days. d. The 24-week exposure was DM 1.56 mg / kg group, DM 3.12 mg / kg group, DM 6.25 mg / kg group and control group, respectively. The continuous exposure for 5 days stopped for 2 days and the exposure time was 24 weeks. The levels of malondialdehyde (MDA), nitric oxide (NO), nitric oxide synthase (NOS), catalase (CAT), glutathione peroxidase (CSH-PX) , Superoxide dismutase (SOD), total antioxidant capacity (T-AOC) activity. Results Compared with the control group, there was no significant difference between the three exposure groups on the 7th day and the control group (P> 0.05). The activities of SOD, CAT, GSH-PX and T-AOC were all decreased in the 24-week exposure groups compared with those in the control group, P <0.05 was statistically significant and the dose-dependent trend. CONCLUSION DM can cause changes of MDA, NO, CAT, T-AOC, CSH-PX and SOD in rat liver after long-term ingestion, resulting in decreased antioxidant capacity and oxidative damage to the liver.