论文部分内容阅读
目的:初步探讨肝干细胞在不同培养条件下的生长情况并探索不同浓度的FGF4对肝干细胞生长的影响。方法:卵圆细胞活化动物模型是利用改良Solt-Farber法建立的:三分之二的肝切除(2/3PH)及服用2-乙酰氨基芴(AAF)造成卵圆细胞活化增殖。在对照组、鼠尾胶组及cytodexTM3微载体3种培养条件下培养肝干细胞并检测不同FGF4浓度下的肝干细胞生长活性。结果:在3种不同培养条件下均可见肝干细胞生长,在cytodexTM3微载体培养瓶中卵圆形细胞增殖速度快,数目增多,形成成片状或串珠状细胞团。结论:cytodexTM3微载体培养方法优于其他方法,有利于肝干细胞生长。
OBJECTIVE: To investigate the growth of hepatic stem cells under different culture conditions and to explore the effects of different concentrations of FGF4 on the growth of hepatic stem cells. METHODS: Animal models of oval cell activation were established using a modified Solt-Farber method: Two-thirds of liver resections (2/3 HP) and 2-acetamido fluorene (AAF) resulted in oval cell activation and proliferation. Liver stem cells were cultured in three culture conditions: control, rat tail gel and cytodexTM3 microcarriers, and liver stem cell growth activity at different FGF4 concentrations was measured. RESULTS: Hepatic stem cell growth was observed under three different culture conditions. The proliferation of oval cells in cytodexTM3 microculture bottles was rapid and increased in number to form flaky or beaded cell clusters. Conclusion: The cytodexTM3 microcarrier culture method is superior to other methods and is conducive to the growth of hepatic stem cells.