论文部分内容阅读
目的了解和掌握温州市首例X群流行性脑脊髓膜炎病例中分离到的脑膜炎奈瑟菌病原学特征。方法对标本进行常规培养和鉴定后,利用血清学和荧光定量PCR方法鉴定分群,采用K-B纸片扩散法进行体外药敏实验。结果该病例证实为X群脑膜炎奈瑟菌引起的脑膜炎病例。采用荧光定量PCR方法对该菌株进行分群,结果与血清学鉴定一致。该菌株对替卡西林、呱拉西林、氨苄青霉素、阿莫西林、头孢噻吩、头孢噻肟、先锋必、美罗培南、红霉素、强力霉素、氧氟沙星、利福平12种抗生素敏感;对萘啶酸、吡咯酸、甲氧苄胺嘧啶、新诺明、多黏菌素B、万古霉素则耐药。结论该病例为温州市首例X群流脑,荧光定量PCR方法对流脑菌株进行分群具有一定的意义,同时应关注流脑新血清群的耐药性变化趋势。
Objective To understand and master the etiological characteristics of Neisseria meningitidis isolated from the first case of epidemic meningitis in Group X in Wenzhou. Methods After routine culture and identification of the specimens, serological and fluorescent quantitative PCR methods were used to identify and classify the specimens. K-B disk diffusion method was used to conduct drug susceptibility tests in vitro. Results The case was confirmed as a case of meningitis caused by N. meningitidis group X. Fluorescent quantitative PCR method was used to cluster the strain and the results were consistent with serological identification. The strain is resistant to 12 kinds of antibiotics including ticarcillin, gentamicin, ampicillin, amoxicillin, cefalotin, cefotaxime, vanguard, meropenem, erythromycin, doxycycline, ofloxacin and rifampicin Sensitive; nalidixic acid, pyrrole acid, trimethoprim, cotrimoxazole, polymyxin B, vancomycin is resistant. Conclusions This case is the first case of X-group meningitis and fluorescence quantitative PCR in Wenzhou City, which is of significance to colonize meningococcal strains. At the same time, we should pay attention to the change trend of drug resistance in meningococcal neoplasia.