造血微环境的研究(五):在照射后的造血再生期,小鼠骨髓及脾脏中氨基葡聚糖含量的变化

• 作 者: 鞠桂芝
• 出 处: 国外医学(放射医学分册)
• 发表日期: 1983年01期
• 关键词: 葡聚糖 脾脏 造血微环境 造血器官 小鼠骨髓 化学法测定 基质细胞 小鼠脾脏 全身照射 造血干细胞

自1972年McCuskey提示氨基葡聚糖(GAG)可能与造血调节有关以来,迄今尚无关于GAG源于造血器官中的何种细胞的资料。本实验用生物化学法测定了照射后小鼠脾脏和骨髓中的GAG的含量,以观察一定条件下的造血干细胞对GAG总量的影响。并通过对受照小鼠的再建骨髓的研究,获得有关基质细胞和造血细胞间的相互作用对造血环境中的GAG水平产生何种影响的资料。结果表明,在850拉德X射线全身照射的雄性小鼠,在照射后第一天,脾脏中的GAG总量从480减至197微克/克干重。7天时增至900微克/克,11天又低

Since 1972, McCuskey suggested that aminoglucan (GAG) may be related to the regulation of hematopoiesis, so far there is no information on which cells in the hematopoietic organs of the GAG. In this experiment, the content of GAG in the spleen and bone marrow of mice after irradiation was measured by biochemistry to observe the effect of hematopoietic stem cells on the total amount of GAG under certain conditions. And by obtaining data on the effects of interactions between stromal cells and hematopoietic cells on GAG levels in the hematopoietic environment by studying the reconstituted bone marrow in irradiated mice. The results showed that in male mice irradiated with 850 Radian X-rays, the total amount of GAG in the spleen was reduced from 480 to 197 μg / g dry weight on the first day after irradiation. Increased to 900 μg / g on day 7 and low on day 11