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目的:检测Notch1和PTEN在正常宫颈组织、宫颈上皮内瘤样病变(CIN)和宫颈癌组织中表达情况,并探讨两者在宫颈癌中表达的临床意义。方法:采用免疫组织化学染色方法(S-P法)检测50例宫颈癌、31例CIN和20例正常宫颈组织中Notch1和PTEN的表达情况。结果:Notch1和PTEN表达定位于细胞浆和细胞核,其中Notch1在宫颈癌中阳性表达率为84.%,明显高于两者在CIN组和正常宫颈组织的表达率,PTEN在宫颈癌的阳性率为32%,低于CIN组和正常宫颈组织的表达率。两者在宫颈癌的表达情况与有无伴有淋巴结转移、临床分期及病理分期有关,其差异有统计学意义(x2=7.948,x2=5.082,x2=7.317;P<0.05);在宫颈癌组织中Notch1和PTEN两者之间比较表达呈负相关(r=-0.402,P<0.05)。结论:Notch1与PTEN的表达失调可能与宫颈癌的发生、发展相关,可做为评价宫颈癌预后的指标。
Objective: To detect the expression of Notch1 and PTEN in normal cervical tissue, cervical intraepithelial neoplasia (CIN) and cervical cancer, and to explore their clinical significance in cervical cancer. Methods: The expressions of Notch1 and PTEN in 50 cases of cervical cancer, 31 cases of CIN and 20 cases of normal cervical tissue were detected by immunohistochemical staining (S-P method). Results: The expression of Notch1 and PTEN was localized in the cytoplasm and nucleus. The positive expression rate of Notch1 in cervical cancer was 84.%, which was significantly higher than that in Notchl and normal cervical tissues. The positive rate of PTEN in cervical cancer Was 32%, lower than that of CIN group and normal cervical tissue. There were significant differences between the two groups in the expression of cervical cancer and with or without lymph node metastasis, clinical stage and pathological stage (x2 = 7.948, x2 = 5.082, x2 = 7.317; P <0.05) There was a negative correlation between the expression of Notch1 and PTEN (r = -0.402, P <0.05). Conclusion: The aberrant expression of Notch1 and PTEN may be related to the occurrence and development of cervical cancer, which may be used as an index to evaluate the prognosis of cervical cancer.