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研究了大鼠脑、心、肝、脾和肾中Ｈｓｐ６０基因表达的积累动力学和恢复动力学规律，同时发现了大鼠等恒温动物在高温应激期间的体温变化具有四个时相（快速升高期、缓慢升高期、缓慢降低期和回升期）的规律。在积累动力学研究中，发现大鼠不同脏器表达Ｈｓｐ６０具有以下规律：脑组织中Ｈｓｐ６０呈现出１５ｍｉｎ内快速升高而后迅速降低并稳定在很低的水平；心和肾组织中，Ｈｓｐ６０呈现出７５ｍｉｎ内随受热时间延长而升高的趋势，其后缓慢下降并均高于对照组，且在脾组织中，Ｈｓｐ６０也呈现出６０ｍｉｎ内缓慢升高而后缓慢降低的变化；肝脏组织中，Ｈｓｐ６０呈现出积累量在应激组均低于对照组且表现出在热应激４５ｍｉｎ内升高而后缓慢降低的趋势。在恢复动力学研究中发现：（１）在脑组织中，Ｈｓｐ６０含量在１０ｈ的恢复期间呈递增趋势（１０ｈ恢复达最高值，为对照组的４倍左右），尔后略为下降；（２）心、肝和脾组织中，Ｈｓｐ６０变化均呈现４ｈ内逐渐减少而后缓慢升高的趋势，分别在１０ｈ、８ｈ、１０ｈ达最大值，在肾组织中Ｈｓｐ６０含量变化也有类似现象（６ｈ内缓慢降低而后快速升高并在恢复１０ｈ左右达最大值），其主要不同点是在恢复前８ｈ内变化幅度较小；（３）在心组织
The accumulation kinetics and recovery kinetics of Hsp60 gene in brain, heart, liver, spleen and kidney of rats were studied. At the same time, it was found that the body temperature changes of rats and other isothermal animals with four phases Rising period, slow rising period, slowly decreasing period and rising period). Accumulation of kinetic studies found that different organs of rats Hsp60 expression has the following rules: Hsp60 brain tissue showed rapid increase within 15min and then rapidly decreased and stabilized at a very low level; heart and kidney tissue, Hsp60 showed Hsp60 also showed a slowly increasing within 60min and then slowly decreasing; in the liver tissue, Hsp60 presented in the liver tissue The cumulative amount of stress in the stress group were lower than the control group and showed a 45min heat stress increased and then decreased slowly. In the study of recovery kinetics, we found: (1) In the brain tissue, the content of Hsp60 showed an increasing trend during the recovery period of 10h (the highest value recovered at 10h, which was about 4 times of the control group), then decreased slightly; (2) , The changes of Hsp60 in liver and spleen tissues all showed a trend of decreasing gradually within 4h and then increasing slowly, reaching the maximum value at 10h, 8h and 10h respectively. There was also a similar phenomenon in Hsp60 content in the kidney (slow decrease within 6h and then fast Rise and reached the maximum value at about 10h after recovery), the main difference is that the change range is small within 8h before recovery; (3)