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Type 2 cytokines are usually predominant in tumor patients and associated with tumor progression.To explorewhether reversing of type 2 predominance could be a promising strategy in tumor immunotherapy,PBMCs of 35lung cancer patients and 19 healthy subjects were prepared and subjected to be examined for cytokine secretionand gene expression.Tetra-Methylpyrazine (TTMP),extracted from a traditional Chinese medicinal herb whichhas been used in clinic to reverse the Th2 status of cancer patients in China,was added to PBMC culture.Determined by RT-PCR,the positive percentages of mRNA expression of type 1 cytokines (8.6% for IFN-γ and11.4% for IL-2) were lower than those of type 2 cytokines (71.4% for IL-4,60% for IL-6 and 80% for 1L-10) inpatients’ PBMCs.The potential of gene expressing (measured as relative intensity to the ratio of β-actin) in thepatients for type 1 cytokines was also in a low level (0.111 for IFN-γ,0.119 for IL-2) in comparison with a relativehigh level for type 2 cytokines (0.319 for IL-4,0.303 for IL-6 and 0.377 for IL-10).Meanwhile,both positivepercentage and relative intensity of gene expression were lower for a type 1 cytokine-related transcription factorT-bet (31.4% and 0.142,respectively) than those for type 2 cytokine-related GATA3 (85.7% and 0.378,respectively).The blood serum levels of IFN-γ and IL-2 in the patients were slightly lower but not significantlywhen compared with healthy control.In contrast,the levels IL-4 and IL-6 in patients were significantly higherthan those in healthy subjects by ELISA analysis.TTMP could enhance supernatant concentration and geneexpression levels of IFN-γ,IL-2 and T-bet,but reduced those of type 2 cytokines.These results demonstrate thatthe lung cancer patients had a predominant expression of type 2 cytokines and TTMP could reverse the type 2dominant status,which might offer an alternative therapeutic regime for lung cancer patients.Cellular &Molecular Immunology.2004;1(1):63-70.
Type 2 cytokines are usually predominant in tumor patients and associated with tumor progression. To explore whether reversing of type 2 predominance could be a promising strategy in tumor immunotherapy, PBMCs of 35 lung cancer patients and 19 healthy subjects were prepared and subjected to be examined for cytokine secretionand gene expression.Tetra-Methylpyrazine (TTMP), extracted from a traditional Chinese medicinal herb which has been used in clinic to reverse the Th2 status of cancer patients in China, was added to PBMC culture. Determined by RT-PCR, the positive percentages of mRNA expression of type 1 cytokines (8.6% for IFN-γ and 11.4% for IL-2) were lower than those of type 2 cytokines (71.4% for IL-4, 60% for IL-6 and 80% for 1L-10 ) inpatients’ PBMCs.The potential of gene expressing (measured as relative intensity to the ratio of β-actin) in thepatients for type 1 cytokines was also in low level (0.111 for IFN-γ, 0.119 for IL-2) in comparison with a relativehigh level for type 2 cyto kines (0.319 for IL-4, 0.303 for IL-6 and 0.377 for IL-10). While, both positivepercentage and relative intensity of gene expression were lower for a type 1 cytokine-related transcription factor T- bet (31.4% and 0.142, respectively) than those for type 2 cytokine-related GATA3 (85.7% and 0.378, respectively). The blood serum levels of IFN-γ and IL-2 in the patients were slightly lower but not significantly compared with healthy control. Levels IL-4 and IL-6 in patients were significantly higherthan those in healthy subjects by ELISA analysis. TTMP could enhance supernatant concentration and gene expression levels of IFN-γ, IL-2 and T-bet, but reduced those of type 2 cytokines. These results demonstrate that the lung cancer patients had a predominant expression of type 2 cytokines and TTMP could reverse the type 2domin status, which might offer an alternative therapeutic regime for lung cancer patients. Cellular & Molecular Immunology. 2004; 1 (1): 63-70 .