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本项研究是以抗杨叶枯病 (Alternariaalternata (Fr.)Keissler)美洲黑杨 (PopulusdeltoidesMarsh .)(♀ )与感病的青杨 (P .cathayanaRehd .) (♂ )及它们的种间杂种F1代和F2 代为材料 ,经室内外人工接种表型抗性鉴定 ,认为美洲黑杨对杨叶枯病的抗性Ala是由 1对隐性纯合基因控制。为进一步识别与Ala抗性基因位点相连锁的标记 ,我们采用RAPD分子标记方法与BSA(BulkedSegregantAnalysis)分群法结合 ,用 4 0 0个 1 0 mer随机引物 ,分析了大约 4 2 0 0条DNA片断 ,鉴别出与Ala位点紧密相连的分子标记 2个 (RPH1 2 6和RPH1 2 4 )。Ala抗病基因位于第 3连锁群 ,2个标记距Ala基因遗传距离均为 3 60cM。研究结果为抗杨叶枯病品种早期鉴定和分子标记辅助抗病育种提供了依据
In this study, we used Populus deltoidesMarsh. (♀) and P. CathayanaRehd. (♂) which are resistant to the leaf blight of Blight (Alternaria alternata (Fr.) Keissler) and their interspecific hybrid F1 The generation F2 and F2 were used as materials to identify the phenotypic resistance of artificial Populus tomentosa infection by indoor and outdoor artificial inoculation, and Ala was controlled by a recessive homozygous gene. To further identify the marker linked to the Ala resistance gene locus, we used the RAPD molecular marker method in combination with the BSA (Bulked Segreganal Analysis) grouping method, and analyzed about 420 DNAs using 400 random primers Two of the molecular markers closely linked to the Ala site were identified (RPH1 2 6 and RPH1 2 4). The Ala resistance gene was located in the third linkage group, and the genetic distance of two markers from Ala gene was 3 60cM. The results provide the basis for the early identification and molecular marker-assisted breeding of disease-resistant blight