急性淋巴细胞白血病p16基因缺失与端粒酶活性关系的研究

• 作 者: 罗绍凯 许多荣 洪文德
• 机 构: 中山医科大学附属第一医院血液科!广东广州510080,中山医科大学附属第一医院血液科!广东广州510080,中山医科大学附属第一医院血液科!广东广州510080
• 出 处: 中国医师杂志
• 发表日期: 2001年S1期
• 关键词: p16基因 端粒重复扩增 基因缺失 缺失率 基因纯合 活性关系 原发性恶性肿瘤 细胞病理学 银染色 染色体末端

目的　探讨急性淋巴细胞白血病 (ALL)p16基因纯合缺失与端粒酶活性之间是否存在一定关系。方法　采用端粒重复扩增法 (TRAP ,telomericrepeatamplificationprotocol)和半定量多重PCR检测了 15例正常人和58例ALL病人端粒酶活性与p16基因缺失情况。结果　发现在ALL中 ,端粒酶活性阳性率为 58 6% ( 34 / 58) ,p16基因缺失率为 39 6% ( 2 2 / 58)。ALL病人端粒酶阳性组及端粒酶阴性组p16基因缺失率分别为 55 9%、12 5% ,两组比较有显著差异 (P <0 0 5)。结论　ALL中p16基因缺失与端粒酶活性之间存在一定关系。

Objective To investigate whether there is a certain relationship between homozygous deletion of p16 gene and telomerase activity in acute lymphoblastic leukemia (ALL). Methods The telomerase activity and deletion of p16 gene in 15 normal subjects and 58 ALL patients were detected by TRAP, telomeric repeata- tion protocol and semi-quantitative multiplex PCR. Results The positive rate of telomerase activity was 58 6% (34/58) in ALL, and the deletion rate of p16 gene was 396% (22/58). The deletion rate of p16 gene in ALL patients with telomerase-positive group and telomerase-negative group was 55.9% and 125%, respectively. There was a significant difference between the two groups (P < 0.05). Conclusion There is a certain relationship between deletion of p16 gene and telomerase activity in ALL.