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为了探讨鸭CD36基因表达对前列腺癌PC3细胞增殖的效应,通过克隆鸭CD36基因CDS区,构建真核表达载体pEGFP-N3+CD36+His,空载体pEGFP-N3为对照,转染培养的前列腺癌PC3细胞。结果表明:前列腺癌PC3细胞均被pEGFP-N3+CD36+His和pEGFP-N3成功转染,转染效率均较高;通过检测转染48 h的CD36+His-tag融合蛋白表达量和细胞数量,CD36+His-tag融合蛋白表达量为98.81 pg/m L,pEGFP-N3+CD36+His组细胞数为0.72×10~6个,pEGFP-N3组细胞数为0.61×10~6个,pEGFP-N3+CD36+His组细胞数显著高于pEGFP-N3组。研究结果揭示,鸭CD36基因的表达对前列腺癌PC3细胞增殖和生长可能有促进作用。
To investigate the effect of duck CD36 gene on the proliferation of prostate cancer PC3 cells, the eukaryotic expression vector pEGFP-N3 + CD36 + His was constructed by cloning the CDS region of duck CD36 gene and the vector pEGFP-N3 was transfected into cultured prostate cancer PC3 cells. The results showed that PC3 cells were successfully transfected with pEGFP-N3 + CD36 + His and pEGFP-N3. The transfection efficiency of PC3 cells was high. The expression of CD36 + His-tag fusion protein and cell number , The expression of CD36 + His-tag fusion protein was 98.81 pg / m L, the number of cells in pEGFP-N3 + CD36 + His group was 0.72 × 10-6, the number of cells in pEGFP-N3 group was 0.61 × 10-6, The number of cells in -N3 + CD36 + His group was significantly higher than that in pEGFP-N3 group. The results revealed that duck CD36 gene expression may promote prostate cancer PC3 cell proliferation and growth.