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用Maximow双盖片法对新生B_6C_3鼠的小脑进行总数达160多片的组织—器官型培养,在普通光镜及明相差显微镜下对髓鞘的生长过程作动态观察的分析。发现在培养10—14天开始有髓鞘形成,至培养16天已明显呈现,并可维持到一个月或更久。结合改良Holmes银染及扫描电镜、透射电镜的观察,为髓鞘体外观察提供模型。作者分析了影响髓鞘体外培养的各种因素,其中培养液内血清的质与量均有密切关系。从而为在国内具体条件下取得成功培养提供参考。
Using Maximow double-cover method, the total number of newborn B_6C_3 mice cerebellum was up to more than 160 tissue-organ culture. The dynamic observation of myelin growth was carried out under ordinary light microscope and phase contrast microscope. Myelination was found to begin at 10-14 days of culture and was evident until 16 days of culture and could be maintained for one month or more. Combined with improved Holmes silver staining and scanning electron microscopy, transmission electron microscopy observation, to provide a model for myelin in vitro observation. The author analyzed various factors affecting myelin in vitro culture, in which the quality and quantity of serum in culture medium are closely related. Thus providing a reference for the successful cultivation under the specific conditions in China.