论文部分内容阅读
目的了解小学生携带甲氧西林耐药金葡菌(MRSA)情况及菌株中毒素基因,为靶向性药物研发等医学实践提供遗传标志物资料。方法方便整群抽取广州市1所小学在校健康小学生1 012名,进行鼻拭子采样,采用PCR实验检测菌株中毒素基因。结果小学生中金葡菌携带率为40.1%,MRSA携带率为1.2%。金葡菌中hlb,sak,scn,chp,sep基因的检出率较高(81.7%,97.8%,82.4%,77.0%),其次是seA,seB,seC基因(33.4%,15.1%, 7.2% )。MRSA菌株中的chp,seB,seC,pvl基因的检出率均明显高于甲氧西林敏感金葡菌(MSSA)菌株(P值均<0.05);MRSA菌株中同时检出2种或3种毒素基因的检出率均明显高于MSSA菌株(P值均<0.05)。结论健康小学生金葡菌及其毒素基因携带率较高,MRSA菌株的潜在致病能力不容忽视。
Objective To understand the primary school students carrying methicillin-resistant Staphylococcus aureus (MRSA) and the strain toxin genes, to provide genetic markers for medical practice such as targeted drug development. Methods A total of 1 012 healthy primary school students in a primary school in Guangzhou were collected for nasal swab sampling and PCR to test the toxin genes in the strains. Results The prevalence of Staphylococcus aureus was 40.1% in primary school students and 1.2% in MRSA. The detection rates of hlb, sak, scn, chp and sep in Staphylococcus aureus were high (81.7%, 97.8%, 82.4%, 77.0%), followed by seA, seB and seC (33.4%, 15.1%, 7.2 %). The detection rates of chp, seB, seC and pvl genes in MRSA were significantly higher than those in methicillin-sensitive Staphylococcus aureus (P <0.05). Two or three The detection rate of toxin genes were significantly higher than that of MSSA strains (all P <0.05). Conclusion The prevalence of S. aureus and its toxin genes in healthy primary school students is high, and the potential pathogenicity of MRSA strains should not be overlooked.