论文部分内容阅读
来自水稻黄单胞菌的harpin蛋白质Hpa1有促进植物生长、诱导植物抗病性的功能,Hpa1序列10~42氨基酸片段(Hpa1_(10-42))的活性比全长分子高1.3~7.5倍。为研究Hpa1_(10-42)在转基因小麦体内表达以后对赤霉病的影响和评价转基因小麦抗病水平与应用潜力,对6个小麦转基因系进行了测定。结果显示,Hpa1_(10-42)在转基因系T_3~T_5代呈现稳定表达,用禾谷镰刀菌一个分离物进行接种以后,转基因系发生赤霉病的程度较非转基因小麦显著降低,且转基因系T_3~T_5代赤霉病降低的趋势一致。另外,转基因系小麦病害减轻的程度,与在非转基因小麦上使用杀菌剂的效果相当,表明Hpa1_(10-42)转基因表达对小麦赤霉病有防治作用。
The harpin protein Hpa1 from X. aeruginosa has the function of promoting plant growth and inducing plant disease resistance. The Hpa1 sequence of 10 to 42 amino acid fragments (Hpa1_ (10-42)) is 1.3 to 7.5 times more active than the full-length molecule. In order to study the impact of Hpa1_ (10-42) on the development of Fusarium head blight in transgenic wheat and to evaluate its disease resistance and potential application, six wheat transgenic lines were tested. The results showed that Hpa1_ (10-42) was stably expressed in T_3 ~ T_5 transgenic lines. After inoculation with one isolate of Fusarium graminearum, Hpa1_ (10-42) was significantly reduced in transgenic lines than non-transgenic wheat, T_3 ~ T_5 generations of the same tendency to reduce the scab. In addition, the extent of disease alleviation in transgenic lines was comparable to the use of bactericides on non-transgenic wheat lines, indicating that Hpa1_ (10-42) transgene expression has a protective effect on wheat head blight.