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Walker 和 Barber 提出了应用放射性自显影术研究植物根系——土壤界面养分的变化规律后,此方法已发展成为这一领域的一个重要研究手段。60年代国外主要使用~(32)P、~(86)Rb 等β、γ能量大的一些示踪元素来研究根系——土壤界面养分分布。由于射线能量大,贯穿能力相对也较强,示踪元素在土体内不仅土壤表面放射性核素的射线对乳胶能进行感光,而且深层土壤内放射性核素的射线对乳胶也能进行感光,
Walker and Barber proposed the application of autoradiography to study plant root-soil interface nutrient changes, this method has developed into an important research tool in this area. In the 1960s, trace elements such as β, γ energy of ~ (32) P and ~ (86) Rb were mainly used to study the nutrient distribution in the root-soil interface. Due to the high energy of radiation and relatively strong penetrating ability, the tracer element not only can detect the latex of the radionuclide in the soil, but also the radionuclide radiation in the deep soil can also sense the latex,