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用卡介苗治疗肿瘤病人,特别是治疗膀胱浅表癌症病人。使用前先确定是否有毒株,常规的方法是噬菌体的分型,但至少需时间3周。作者介绍了一种新的快速鉴别法,能在24小时内完成,即高效液相色谱法(HPLC)。供试菌为结核杆菌、牛型分枝杆菌和卡介苗等84株。在骆氏培养基上培养后用2ml含25%KOH和50%乙醇皂化,用2ml氯仿提取,结核环脂酸形成了溴苯酰酯,然后上柱,有两种方法:
With BCG treatment of cancer patients, especially the treatment of superficial bladder cancer patients. Before use to determine whether there is a strain, the general method is phage typing, but it takes at least 3 weeks. The authors introduced a new rapid identification method that can be completed in 24 hours, ie High Performance Liquid Chromatography (HPLC). The test bacteria Mycobacterium tuberculosis, mycobacterium bovis and BCG 84. After culturing on lukewarm medium, it is saponified with 2 ml of 25% KOH and 50% ethanol and extracted with 2 ml of chloroform. The tuberculinic acid forms bromophenacyl ester and then on the column, there are two methods: