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目的:考察强碱性还原电位水(ERW)的制备工艺和杀菌效果。方法:利用三槽型离子膜电解槽和自制钛基贵金属电极制备强碱性还原电位水。采用悬液定量杀菌法,利用ERW对大肠杆菌(8099)和枯草杆菌黑色变种芽孢(ATCC9372)进行灭菌实验。结果:在电解质质量浓度为1～5g·L-1,电解时间为10～80min,电流密度为40～100mA.cm-2下,电解质浓度、电解时间和电流密度的增加,会增加ERW的pH值,减少其氧化还原电位(ORP值)。杀菌试验结果表明,10min内,ERW对大肠杆菌的杀菌率达到99.91%,30min内接近100%;60min内ERW对枯草杆菌黑色变种芽孢的杀菌率为94.12%。结论:ERW最佳制备工艺是2～5g·L-1NaCl,电解时间为30～60min,电流密度为60～100mA.cm-2。ERW对大肠杆菌和枯草杆菌黑色变种芽孢具有很强杀灭作用。
Objective: To investigate the preparation process and bactericidal effect of strong alkaline reduction potential water (ERW). Methods: The strong alkaline reduction potential water was prepared by using three-cell ion-exchange membrane electrolyzer and self-made titanium-based noble metal electrode. The suspension sterilizing method was used to sterilize Escherichia coli (8099) and Bacillus subtilis ATCC9372 by using ERW. Results: The increase of electrolyte concentration, electrolysis time and current density at the electrolyte concentration of 1 ~ 5g · L-1, the electrolysis time of 10 ~ 80min and the current density of 40 ~ 100mA · cm-2 increased the pH of ERW Value, reducing its redox potential (ORP value). The bactericidal test results showed that within 10min, the killing rate of ERW to Escherichia coli reached 99.91% and nearly 100% in 30min. The killing rate of ERW to Bacillus subtilis black spores within 60min was 94.12%. Conclusion: The optimal preparation process of ERW is 2 ~ 5g · L-1 NaCl, the electrolysis time is 30 ~ 60min and the current density is 60 ~ 100mA · cm-2. ERW has a strong killing effect on E. coli and B. subtilis black mutants.