Study on Preparation of Temperature-sensitive Injectable Hydrogel

• 作 者: LIU Ling-xiu HU Guo-ying LIU X
• 机 构: Biomedical Engineering Department,Tianjin Institute of Urological Surgery
• 出 处: 中国生物医学工程学报（英文版）
• 发表日期: 2004年期
• 关键词: hyaluronan poly(N-isopropylacrylamide) methylcellulose injectable hydrogel tissu
• 基金项目: The Nattional Key Scientific Program-Nanoscience and Nanotechnology

To get a sort of new scaffold material for soft tissue reconstruction, we have prepared XLHA-PNIPAAm and XLHA-MC injectable hydrogels through blending crosslinked HA(XLHA)and two temperature-sensitive materials differed in degradation poly(N-isopropylacrylamide)(PNIPAAm)and methylcellulose(MC),respectively. We tested the injectablility, enzymatic biodegradability,temperature-sensitivity,structure cytotoxicity and hemolysis of the two injectable hydrogels. Our research has successfully obtained the preparation condition of XLHA-PNIPAAm injectable hydrogel, and verified that adding non-degradable material PNIPAAm can postpone the degradation of HA more effectively than degradable material MC. PNIPAAm prepared with 5 kGy dose radiation,MBAAm/NIPAAm(M/M)=0.015, monomer concentration=3% produced XLHA-PNIPAAm with slowest enzymatic biodegradability. DSC results showed that temperature-sensitivity of the XLHA-PNIPAAm was more stable than that of XLHA-MC. Two composite hydrogels were qualified in cytotoxicity and hemolysis tests and the biocompatibility of XLHA-PNIPAAm hydrogel showed better than XLHA-MC hydrogel.