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选择已成功免疫猪瘟(CSF)和猪繁殖与呼吸综合征(PRRS)弱毒疫苗的仔猪作为实验动物,提取其骨髓、胸腺、肺门淋巴结和脾脏,进行T淋巴细胞的分离培养,然后用PRRSV特异性肽GP5-1、GP5-2对培养的细胞进行刺激,再培养至24,48h时间点时,离心取细胞上清液,检测上清液中TGF-β1的分泌含量的变化。结果显示,PRRSV GP5类特异性肽刺激作用后,CSF、PRRS免疫猪组织淋巴细胞中TGF-β1的分泌量减少,表明GP5-1、GP5-2肽具有减弱免疫抑制,恢复宿主抗病毒免疫应答的作用。本试验为PRRSV合成肽疫苗及疫苗佐剂的研制提供了理论依据。
The piglets that have been successfully immunized with classical swine fever virus (CSF) and porcine reproductive and respiratory syndrome (PRRS) attenuated vaccine were selected as experimental animals and their bone marrow, thymus, hilar lymph nodes and spleens were isolated, T lymphocytes were isolated and cultured, and then PRRSV The specific peptides GP5-1, GP5-2 cultured cells were stimulated, and then cultured to 24,48h time point, the supernatant was centrifuged to detect changes in the secretion of TGF-β1 in the supernatant. The results showed that the secretion of TGF-β1 in porcine lymphocytes secreted by CSF and PRRS decreased after stimulation with PRRSV GP5-specific peptides, indicating that GP5-1 and GP5-2 peptides attenuated the immunosuppression and restored the host anti-viral immune response Role. This experiment provides a theoretical basis for the development of PRRSV synthetic peptide vaccine and vaccine adjuvant.