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月季品种繁多,花色丰富,深受人们的喜爱,冠以“花中之后”。但长期以来,月季一直采用嫁接、扦插的方法进行繁殖,繁殖速度慢,不能满足市场需求。现介绍一种月季快速繁殖的新技术——组培育苗。方法:取月季嫩茎做外植体,用MS添加3%的蔗糖和0.7%的琼脂为基本培养基,进行培养。具体做法:①将基本培养基附加6BA0.5mg/l和NAAO.1mg/l配制好后调PH值到5.7-6分装,高压灭菌半小时,取出备用。②外植体采集:从地栽或盆裁月季取其嫩茎,去掉茎上的叶和刺,用自来水冲洗干净。③外植体消毒灭菌:将洗净的嫩茎先用75%酒精处理半分钟,再用0.1%升汞灭菌8分
Rose variety, rich colors, loved by the people, crowned “after the flower ”. But for a long time, rose has been using grafting, cutting methods of reproduction, reproduction slow, can not meet market demand. Now introduce a new technology for rapid propagation of roses - tissue culture seedlings. Methods: The tender stems of Chinese rose were used as explants. MS medium supplemented with 3% sucrose and 0.7% agar was used as the culture medium. Specific approach: ① the basic medium supplemented 6BA0.5mg / l and NAAO.1mg / l formulated PH values to 5.7-6 after dispensing, autoclave for half an hour, remove the spare. ② explant collection: planted or potted rose taken from the tender stems, remove the leaves and thorns on the stem, rinse with tap water. ③ explants disinfection and sterilization: Wash the tender stems with 75% alcohol for half a minute, and then sterilized with 0.1% mercuric chloride 8 points