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[目的]初步研究肥胖候选基因过氧化物酶增殖激活物受体-γ2(PPAR-γ2)基因Plo12Ala的变异以及解偶联蛋白2(UCP2)基因Ala55Val的变异与成都地区人群单纯性肥胖的关系,并考察两者是否存在协同作用,从而为成都地区单纯性肥胖的研究与防治提供参考。[方法]将研究对象分为单纯性肥胖组和对照组。用聚合酶链反应—限制性片断长度多态性的方法(PCR-RFLP)检测两组研究对象的PPAR-γ2、UCP2基因的变异情况,分析两个基因的变异以及联合变异在两组间差异是否具有统计学意义。[结果]UCP2基因的变异在两组中的差异有统计学意义(P﹤0.05);而PPAR-γ2基因变异以及两者的联合变异在两组中的差异无统计学意义(P﹥0.05)。[结论]UCP2基因Ala55Val变异可能与成都地区单纯性肥胖相关,而PPAR-γ2的Plo12Ala基因的变异不是成都地区人群肥胖的危险因素,且两者不具有协同作用。
[Objective] To investigate the relationship between mutation of Plo12Ala gene of peroxisome proliferator-activated receptor-γ2 (PPAR-γ2) gene and Ala55Val of uncoupling protein 2 (UCP2) gene and simple obesity in Chengdu population , And examine whether there is synergy between the two, so as to provide a reference for the study and prevention of simple obesity in Chengdu. [Methods] The subjects were divided into simple obesity group and control group. The variation of PPAR-γ2 and UCP2 genes in two groups were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The differences of two genes and the association between the two variants were analyzed Is statistically significant? [Results] The variation of UCP2 gene in the two groups was statistically significant (P <0.05). However, there was no significant difference in the mutation of PPAR-γ2 and the combination of the two between the two groups (P> 0.05) . [Conclusion] The mutation of Ala55Val in UCP2 gene may be related to simple obesity in Chengdu. However, the variation of Plo12Ala gene in PPAR-γ2 is not a risk factor for obesity in Chengdu population, and the two do not have synergistic effects.