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Objective To study the effects of melatonin on pethidine-induced physical dependence and its antioxidative action.Methods A trauma-pain model was established in Wistar rats by combining right limb amputation with 50 ℃ tail-flick test.The contents of MDA and the activity of the total superoxide dismutase(SOD)in the brain tissue of trauma-pain rats were detected by thiobarbituric acid method and the xanthine oxidase method.A physical dependence model in mice was reproduced by subcutaneous injection of pethidine and the withdrawal syndromes were induced by intraperitoneal injection of naloxone.Results The contents of MDA enhanced,but the activity of SOD decreased greatly in brain tissues postinjury in rats.Melatonin(30,60,120 mg·kg-1)i.p.reduced the contents of MDA and enhanced the activity of SOD dose-dependently.Melatonin alone showed no withdrawal syndrome when it was given until the dosage of 840 mg·kg-1.Moreover,Melatonin(5,15,20 mg·kg-1)obviously inhibited the withdrawal jumpings of mice in pethidine-treated groups dose-dependently(P<0.01),the jumping rates of mice were decreased 61.4%,72.8%,84.8%,respectively.Conclusions The present results indicated that melatonin has good antioxidative effects on the trauma rats.In addiction,melatonin might inhibit withdrawal syndromes in pethidine-dependent mice.
Objective To study the effects of melatonin on pethidine-induced physical dependence and its antioxidant action. Methods A trauma-pain model was established in Wistar rats by combining right limb amputation with 50 ° C tail-flick test. The contents of MDA and the activity of the total superoxide dismutase (SOD) in the brain tissue of trauma-pain rats were detected by thiobarbituric acid method and the xanthine oxidase method. A physical dependence model in mice was reproduced by subcutaneous injection of pethidine and the withdrawal syndromes were induced by intraperitoneal injection of naloxone. Results of the contents of MDA enhanced, but the activity of SOD decreased greatly in brain tissues postinjury in rats. Melatonin (30, 60, 120 mg · kg -1) ipreduced the contents of MDA and enhanced the activity of SOD dose- dependently . Melatonin alone showed no withdrawal syndrome when it was given until the dosage of 840 mg · kg-1.Moreover, Melatonin (5, 15, 20 mg · kg-1) obviously inhibited the withdrawal jumpin gs of mice in pethidine-treated groups dose-dependently (P <0.01), the jumping rates of mice were decreased 61.4%, 72.8%, 84.8%, respectively. Conclusions The present results indicated that melatonin has good antioxidative effects on the trauma rats .In addiction, melatonin might inhibit withdrawal syndromes in pethidine-dependent mice.