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目的了解黑龙江省生鸡肉中沙门菌污染状况及抗生素耐药情况。方法根据《食品安全国家标准食品微生物学检验沙门氏菌检验》(GB4789.4-2010),对黑龙江省880份生鸡肉样品进行检验,并采用微量肉汤稀释法(MIC)对10种抗生素进行药物敏感性试验。结果 880份样品中共检出86株沙门菌,检出率为9.77%。86株沙门菌分属于5个血清型,其中以肠炎沙门菌和印第安纳沙门菌的比例最高。抗生素敏感性试验显示,86株沙门菌对萘啶酸和氨苄西林耐药率最高,分别为95.3%和62.8%;32株对3种及以上抗生素耐药,共产生6种耐药谱,其中2种耐药谱优势明显,分别为AMP-TET-NAL-GEN-SXT-CIP(28.1%,9/32)和AMP-TET-NAL(21.9%,7/32)。结论黑龙江省生鸡肉中沙门菌污染及耐药性均较重,应加强卫生管理和抗生素使用上的管理。
Objective To understand the status of Salmonella contamination and antibiotic resistance in raw chicken meat in Heilongjiang Province. Methods According to the National Food Safety Standard Microbiological Test for Salmonella (GB4789.4-2010), 880 samples of raw chicken meat in Heilongjiang Province were tested, and 10 kinds of antibiotics were tested for drug sensitivity by using micro broth dilution method (MIC) Sexual test. Results A total of 86 strains of Salmonella were detected in 880 samples with the detection rate of 9.77%. 86 strains of Salmonella belong to 5 serotypes, of which the highest proportion of Salmonella enteritidis and Indiana. The results of antibiotic sensitivity test showed that 86 Salmonella strains had the highest rates of nalidixic acid and ampicillin, 95.3% and 62.8% respectively; 32 strains were resistant to 3 or more antibiotics, The two drug-resistant spectrums showed obvious advantages, AMP-TET-NAL-GEN-SXT-CIP (28.1%, 9/32) and AMP- TET-NAL (21.9%, 7/32), respectively. Conclusion The contamination and drug resistance of Salmonella in raw chicken in Heilongjiang Province are all serious, and the management of hygiene management and antibiotic use should be strengthened.