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目的:探讨双根清脑颗粒治疗血管性痴呆的机理。方法:采用梅氏法复制VD大鼠模型。以双根清脑颗粒干预,免疫组化方法检测模型大鼠脑切片BrdU阳性细胞;Moriss水迷宫观察隐匿平台获得时间与大鼠空间搜索时间。结果:Moriss水迷宫实验,双根清脑颗粒组与模型组相比,隐匿平台获得时间明显缩短(P<0.05),大鼠空间搜索次数亦明显增多(P<0.05)。免疫组化检测显示:双根清脑颗粒各组大鼠BrdU阳性细胞表达明显高于模型组(P<0.05)。结论:双根清脑颗粒可改善VD模型大鼠的学习与记忆的功能,其机制可能与促进VD模型大鼠新生神经元增殖有关。
Objective: To investigate the mechanism of Shuanggan Qingnao granule in treating vascular dementia. Methods: The rat model of VD was duplicated by Mei’s method. The intervention of Shuanggen Qingnao granule was used to detect the BrdU positive cells in brain sections of model rats by immunohistochemistry. The acquisition time of the hidden platform and the space search time of rats were observed by Morris water maze. Results: Compared with model group, Shuanggen Qingnao granule group in Moris water maze test showed that the acquisition time of hidden platform was significantly shortened (P <0.05) and the number of spatial search was also significantly increased (P <0.05). Immunohistochemistry showed that the expression of BrdU positive cells in Shuanggen Qingnao granule group was significantly higher than that in model group (P <0.05). Conclusion: Shuanggen Qingnao Granules can improve the learning and memory function of VD model rats, and its mechanism may be related to promoting the proliferation of neonatal neurons in VD model rats.