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目的本课题着重研究自身髌腱中1/3骨－腱－骨重建前交叉韧带后1年移植物内细胞生物学行为的变化,重点观察重建后1年移植物内细胞Ⅲ型胶原的表达.方法通过建立狗自身骸腱中1/3骨－腱－骨重建前交叉韧带的动物模型,采用免疫组化和原位杂交方法检测重建后1年Ⅲ型胶原在重建物中的表达.结果从常规组织学染色看,重建后1年移植物与正常前交叉韧带结构相似;但免疫组化和原位杂交结果显示:正常前交叉韧带内表达Ⅲ型胶原细胞呈规律性分布,而重建后1年移植物中表达Ⅲ型胶原的细胞明显增多.且呈弥散分布.结论自身髌腱中1/3骨－腱－骨重建前交叉韧带后1年,移植物仍然不是一个正常的韧带组织.“,”Objective To compare the morphological changes and cell biological behavior in thenormal anterior cruciate ligament (ACL) and the autograft. Methods Immunohistochemistry and in situ hy- bridizstion were used to detect the expression of type Ⅲ collagen one year after ACL reconstruction in eight dogs.ResultS In normal ACL, the cell that expressed type Ⅲ collagen distributed regUlarly, while in autograftsafter one year, there were more expressions of type Ⅲ collagen, and cells that expressed type Ⅲ collagen distributed irregularly. Conclusion One year after ACL reconstruction with autogenous patellar tendon, the graftstill did not appear to be a normal ligament structure.