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目的:探讨微小RNA(miRNA)-125在正常人和肝癌患者血清,以及肝癌患者正常肝脏组织和肝癌组织中的表达。方法:应用荧光实时定量PCR方法检测miRNA-125,包括miRNA-125a、miRNA-125b在正常人和肝癌患者血清中的含量。同时收集并检测肝细胞癌患者术中取得的正常肝脏组织和癌症组织miRNA-125含量。结果:1)与正常人血清相比,miRNA-125在癌症患者血清中的含量没有明显变化。2)与正常肝脏组织相比,肝癌组织中miRNA-125含量明显下调,有统计学差异(P<0.001)。结论:MiRNA-125,包括miRNA-125a和miRNA-125b在肝癌组织中表达显著减少,但是在肝癌患者血清并没有明显变化。
Objective: To investigate the expression of microRNA (miRNA) -125 in normal and hepatocellular carcinoma patients and normal liver tissues and hepatocellular carcinoma tissues. Methods: The miRNA-125, including miRNA-125a, miRNA-125b in serum of normal and hepatocellular carcinoma patients were detected by real-time fluorescence quantitative PCR. At the same time, we collected and detected the miRNA-125 content in normal liver tissues and cancerous tissues obtained from patients with hepatocellular carcinoma. Results: 1) There was no significant change in the serum level of miRNA-125 in cancer patients compared with normal human serum. 2) The level of miRNA-125 in hepatocellular carcinoma was significantly lower than that in normal liver tissues (P <0.001). Conclusions: The expression of miRNA-125, including miRNA-125a and miRNA-125b, was significantly decreased in HCC tissues, but there was no significant change in the serum of HCC patients.