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用脑内微透析技术结合 HPL C-柱后固定化酶反应器 -电化学检测器方法分析比较了老龄大鼠和年轻成年大鼠额叶皮层乙酰胆碱的释放 ,并探讨了它和学习记忆功能减退的相关性。老龄大鼠额叶皮层乙酰胆碱的基础水平为 ( 0 .15 5 9± 0 .18)pmol/5μl,远较年轻大鼠的 ( 0 .2 93 9± 0 .14 ) pmol/5μl为低。老龄大鼠额叶胆碱能神经元对高 [K+ ]溶液的反应较弱 ,乙酰胆碱的释放量仅增加 3 0 .18% ,而年轻大鼠可增加 96%。老龄大鼠对新异环境刺激的反应也较年轻大鼠为弱 ,但电刺激 Meynert基底核仍能明显提高老龄大鼠额叶皮层乙酰胆碱的释放。实验结果还表明 ,老龄大鼠额叶皮层的乙酰胆碱水平有较大的个体差异 ,9只老龄大鼠额叶皮层乙酰胆碱释放量的变化范围为 0 .0 0 67pmol/5 μl～ 0 .41pmol/5 μl。这种差异也反映在老龄大鼠对被动回避反应的记忆保持能力上 ,2 4h步下潜伏期和乙酰胆碱释放量有较好的相关性 ,相关系数为 0 .86。本研究所获得的实验结果对进一步阐明老龄大鼠认知性功能缺损的机制具有重要意义
The release of acetylcholine in the frontal cortex of aged rats and young adult rats was analyzed by intracerebral microdialysis combined with HPL C-post immobilized enzyme-electrochemical detector and compared with that of learning and memory deficits Relevance. The basal level of acetylcholine in the frontal cortex of old rats was (0.15 5 9 ± 0.18) pmol / 5μl, which was much lower than that of the young rats (0.2393 ± 0.014 pmol / 5μl). The response of the frontal cholinergic neurons to high [K +] solution in aged rats was weak, the release of acetylcholine increased only by 30.18%, while the increase in young rats was 96%. In older rats, the response to neo-isoenvironmental stimulation was also weaker than in younger rats, but electrical stimulation of Meynert’s basal ganglia significantly increased the release of acetylcholine in the frontal cortex of aged rats. The experimental results also showed that the acetylcholine levels in the frontal cortex of the aging rats had large individual differences. The amount of acetylcholine release in the frontal cortex of 9 aged rats ranged from 0. 0 67 pmol / 5 μl to 0.41 pmol / 5 μl. This difference was also reflected in the memory retention of passive avoidance response in aged rats, with a good correlation between incubation latency and acetylcholine release at 24 h, with a correlation coefficient of 0.86. The experimental results obtained in this study are of great significance to further elucidate the mechanism of cognitive impairment in aging rats