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自新生大鼠颅骨分离出的成骨细胞在含５０ｍｇ／Ｌ维生素Ｃ和１０ｍｍｏｌ／Ｌβ－甘油磷酸钠的ＤＭＥＭ＋１０％ＦＣＳ培养液内逐渐出现聚集重叠而形成结节，ＶｏｎＫｏｓｓａ染色证实结节内有钙盐沉积，细胞的碱性磷酸酶染色显示结节的钙化与高活性酶的活动有关。电镜示结节内有大量的钙盐沉积在胶原纤维上，一些类似骨细胞的细胞被包埋在钙化骨质内，整个由细胞、胶原纤维和钙盐组成的三维结构类似于体内膜内成骨。实验结果提示：体外培养的鼠颅骨成骨细胞能形成钙化骨质，进一步证实所分离的细胞具有成骨细胞的性质。
Osteoblasts isolated from the skull of newborn rats gradually gathered and overlapped in DMEM + 10% FCS medium containing 50mg / L vitamin C and 10mmol / L β-glycerophosphate to form nodules. VonKossa staining confirmed the presence of calcium Salt deposition, cell alkaline phosphatase staining showed nodular calcification and activity of highly active enzymes. Electron microscopy nodules have a lot of calcium deposition in the collagen fibers, some cells similar to bone cells are embedded in calcified bone, the entire three-dimensional structure composed of cells, collagen fibers and calcium salts similar to the in vivo membrane Osteogenesis. The experimental results suggest that the calvarial osteoblasts cultured in vitro can form calcified bone, further confirming the osteoblastic nature of the isolated cells.