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以牛血清白蛋白(BSA)为抗原,大剂量(250毫克/公斤体重)一次注入家兔耳静脉内,造成动物“一过性”血清病。隔日采集静脉血,分离血清于-40℃冻存,在统一条件下用聚乙二醇沉淀-补体消耗试验检测家兔循环免疫复合物(CIC)的动态,并用CH_(50)试验平行测定血清中总补体量。实验结果表明:家兔于注射BSA抗原后第6天开始检出CIC,第10天达最高峰,第12天下降;血清中补体含量与此相反,第6天开始下降,第10天下降到最低水平,第12天后又逐渐回升。本法检测家兔急性实验性血清病CIC较为适用。
Bovine serum albumin (BSA) as antigen, a large dose (250 mg / kg body weight) into the rabbit ear vein, resulting in animal “transient” serum disease. Venous blood was collected on the next day, the serum was separated and frozen at -40 ° C. The dynamic changes of rabbit immune complex (CIC) were detected by polyethylene glycol precipitation - complement depletion test under uniform conditions. The serum levels of serum The total amount of complement. The results showed that CIC began to be detected on the 6th day after injection of BSA antigen in rabbits, reached the peak on the 10th day and decreased on the 12th day. In contrast, the serum complement content decreased from the 6th day to the 10th day The lowest level, the first 12 days and then gradually picked up. This method is more suitable for detecting CIC in rabbits with acute experimental serum disease.