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本文提出了一种培基不需高压蒸气灭菌,实验器材,水等不用灭菌,不用无菌操作的布氏菌选择性培基,在一般实验室内,能有效地抑制杂菌,并有促进布氏菌生长等作用。此选择性培养基的配方是在100毫升马铃薯基础培基中加入制霉菌素1～2毫克、多粘菌素B100～1,000单位、杆菌肽300～3,000单位、乙基紫1/500万～1/83万。上述加入的四种药物有协同抑菌作用,但浓度过高它们的抑制杂菌的效果不一定就更好。以浓度偏低的为宜。经试
This paper presents a culture medium without autoclave sterilization, laboratory equipment, water and other non-sterile, non-sterile operation of Brucella selective culture medium, in general laboratories, can effectively inhibit the bacteria, and Promote the growth of brucella and other effects. The selective medium is prepared by adding 1 to 2 milligrams of nystatin, 100 to 1,000 units of polymyxin B, 300 to 3,000 units of bacitracin and 1/500 to 1 of ethyl violet on a basic culture medium of 100 ml potato / 830,000. The four drugs added above have a synergistic bacteriostatic effect, but their effect on inhibiting bacteria is not always better. Low concentration is appropriate. After the test