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探讨用逆转录病毒表达载体携带反义核酸进入 ＣＭＬ细胞并作用于 ｂｃｒ－ａｂ１基因的方法。方法：反义序列用ＲＴ－ＰＣＲ法，取自Ｋ５６２细胞系，使之成功地构建了正反义ＲＮＡ的逆转录病毒表达载体。结果：与正义链相比，反义ｂｃｒ－ａｂ１序列显著抑制Ｋ５６２及ＣＭＬ细胞生长，其抑制程度与剂量呈正相关，其细胞形态学改变支持其进入凋亡进程。结论：此反义核酸是探索ＣＭＬ基因治疗的重要材料。
To investigate the use of retroviral expression vectors to carry antisense nucleic acids into CML cells and act on the bcr-ab1 gene. Methods: Antisense sequences were obtained from K562 cell line by RT-PCR method. The retroviral expression vector of antisense RNA was successfully constructed. RESULTS: Compared with the sense chain, antisense bcr-ab1 sequence significantly inhibited the growth of K562 and CML cells, and the degree of inhibition was positively correlated with the dose. The morphological changes of the bcr-ab1 sequence supported its entry into apoptosis. Conclusion: This antisense nucleic acid is an important material for exploring CML gene therapy.